Mesothermal lode-gold deposits often pose problems during sampling because of their complex and erratic gold grade distribution, and the presence of coarse gold. Effective sampling and sample preparation protocols must form the basis of any resource estimate at whatever stage a project is at. Design of a sampling regime must be based upon a thorough understanding of orebody mineralogy and geology. This should include consideration of gold particle size and distribution. The sample type, size and density employed must also be based on the underlying geology of the deposit. Choice of sample preparation procedure and assay type is of critical importance if fundamental sampling error and analytical errors are to be minimised. Traditional fire assays, using small charge sizes, consistently understate assays gained from large charge sizes and techniques such as screen fire assay and bulk leach extractable gold assay. The proportion of coarse gold within samples will have a profound effect on the requirements for sample preparation and assay method. The application of Gy sampling theory can help to reduce errors within the processing of samples of broken rock. Quality control/assurance of sampling regimes must also be critically assessed. The problems associated with sampling mesothermal lode-gold type deposits are presented as case studies from Western Australia and North America.