Bacterial leaching of museum grade chalcopyrite and chalcopyrite concentrates at low sorptive relative humidities (55-91 per cent rH) was demonstrated. Optimum release rates were 13 and 27 mg/gram/100 days copper (Cu++) at 91 per cent and 80 per cent rH and 12 and 33 mg/gram/100 days iron (Fe++) at 80 per cent and 73 per cent rH for concentrate and "pure" chalcopyrite respectively. The optimum copper release rates occurred between 73 per cent - 86 per cent rH and 30-40 mM acid concentrations. Bacterial copper leaching rates were some twenty times slower for emersed copper concentrates, at low relative humidity, compared with submerged cultures. Bacterial copper solubilization was almost quantitatively inhibited above 150 mM acid levels (< pHO.8).