Experiments conducted at Tahmoor Colliery, Sydney Basin, have demonstrated the variability in chemical and isotopic composition of seam gases issuing from boreholes in the Bulli seam and desorbing from lump coal from the same source.From this work it appears that an immediate preferential release of methane accompanies the physical disturbance of the coal seam by penetration with development or drilling or by detachment. In addition, both early released methane and carbon dioxide are depleted in 13C relative to later desorbed gases, therefore the analyses of gas samples taken after such events may not reflect the true bulk composition of the undisturbed seam gas in situ. A method for overcoming this problem by taking gas samples at depth in the "steady state" is proposed.