There is a growing demand for copper driven by its applications in renewable energy and electric vehicles. Sulfide ores are an important source of copper. Flotation is a critical front-end process used to remove gangue minerals and concentrate the copper minerals. The electrochemical activity of sulfide minerals contributes to their overall activity in the flotation pulp. The mineral rest potential dictates how collectors interact with the mineral surface, and hence ultimately on the flotation response. Dissolved oxygen, (DO), pH, redox potential (Eh) and water composition and ionic strength are also significant in controlling flotation performance. The aim of this investigation was to determine the influence of the electrochemical environment both during milling and flotation on the flotation behaviour of bornite and chalcocite. For this investigation, the interactions of collector with bornite and chalcocite were studied using synthetic plant water (SPW1) and deionised water (DIW) at 5 different pH levels. Sodium isobutyl xanthate (SIBX) was used both in microflotation, collector adsorption and zeta potential tests.