Sinter quality depends heavily on the mineral phases, textures and physical structure of the sinter, which are formed upon solidification of the sinter melt. The interaction of sinter initial melt with its surrounding materials will impact the properties of the sinter melt and consequently the solidified structure and quality of resultant sinter. Spreading and penetration are two important phenomena dictating the interaction of the initial melt with its surrounding materials, such as nuclei and adhering fines. In the present study, the penetration behaviour of an initial melt into various natural iron ore substrates of different ore types during sintering was studied using a laboratory scale penetration testing procedure. The penetration depth and width were found to increase with temperature and vary with the type of iron ore due to their unique pore structure and mineralogical texture.