Quantification of site-specific sulfide oxidation rates is crucial to accuracy of prediction of, for example, the environmental impact of a mine site. Sulfide oxidation reactions are reportedly increased by bacterial mediation; determination of abiotic and biotic weathering rates thus requires the establishment of sterile control samples. However, sterilisation often involves exposure of samples to extreme conditions that may alter the physical, chemical, or mineralogical characteristics of the tailings and hence affect element release rates. The impact of various sterilisation techniques, common and novel, on the element release rates from tailings from the Kristineberg site, northern Sweden, has been investigated. Batch weathering experiments indicated that the different methods of sterilisation (autoclaving, heat treatment, ethanol/heat treatment, application of antibiotics, rinsing with distilled water, and -radiation) did not greatly affect long-term element release rates. Long-term experiments (>30 days) are thus to be recommended for establishment of weathering rates. Sterilised and untreated control samples showed similar element release rates after ~100 days of experiments; possible explanations include that microbial oxidation is not important under our experimental conditions (Po2 = 0.2 atm, pH ~2.3, 22C, 0.01 M HNO3, no additional nutrients).